Qualitative and quantitative detection of aldehydes in DNA with 2-amino benzamidoxime derivative

An aldehyde-reactive probe based on 2-amino benzamidoxime (ABAO) framework was introduced, which can selectively label aldehydes in DNA through intramolecular ring closure under mild aqueous solutions. We screened ABAO derivatives that can undergo a cyclization with the formylated nucleobases to generate a fluorescence nucleoside, and of these derivatives 5-methoxy-ABAO (PMA) emerged as the optimal choice. PMA can sensitively and selectively react with 5fU, 5fC and AP to form fluorogenic dihydroquinazoline derivatives, which also can quantify DNA damages induced by γ -irradiation. PMA-initiated labeling strategy provides great convenience for qualitative and quantitative detection of aldehydes in DNA. An aldehyde-reactive probe based on 2-amino benzamidoxime (ABAO) framework was introduced, which can selectively label aldehydes in DNA through intramolecular ring closure under mild aqueous solutions. PMA can sensitively and selectively react with 5fU, 5fC and AP to form fluorogenic dihydroquinazoline derivatives, which also can quantify DNA damages induced by γ -irradiation.