Molecularly imprinted ratiometric fluorescence detection of tetracycline based on its fluorescence enhancement effect caused by tungsten trioxide quantum dots

This paper reports a novel probe developed based on the tungsten trioxide quantum dots (WO3-x QDs) and molecularly imprinted polymers for the detection of trace tetracycline (TC) in the complex food matrix. Tungsten ion (W6+) in WO3-x QDs has a fluorescence enhancement effect on TC, and TC has a fluorescence quenching effect on WO3-x QDs. The blue emission of the WO3-x QDs (λem = 470 nm) as a reference and the yellow emission of the TC (λem = 550 nm) as a response were utilized for the ratiometric fluorescence detection. In order to improve its selectivity, the molecular imprinting technology was combined to construct molecularly imprinted ratiometric fluorescent probes (MIRFPs). Therefore, the MIRFPs can not only selectively detect TC, but also realize the visual detection from blue to yellow. Under the optimal conditions, the linear ranges of 0.01 ∼ 10.0 μmol/L and 20.0 ∼ 80.0 μmol/L were obtained with the limits of detection of 3.23 nmol/L and 6.37 μmol/L, respectively. Furthermore, the MIRFPs had been successfully applied to the detection of TC in milk and eggs. The satisfactory recoveries were in the range of 92.7 ∼ 102.9 % with relative standard deviations (RSD, n = 3) below 1.59 %. This work offers a good strategy for the detection of food hazards.