Introduction: Amalaki, Ashwaghandha and Lakshmanphal are the three common rejuvenating drugs which has been used treatment, due to its high phenols and antioxidant activity hence study conducted the analysis total phenols content in above samples. Materials & Methods: Aims & Objectives: Estimation of total phenolic content, Study setting: ACRL Laboratory SJGAMC&H Koppal. Total phenolic content using UV Visible spectrophotometer the amounts of phenols were determined by Folin Ciocalteau reagent method, Gallic acid is used as standard and total phenols were expressed as mg/g equivalent to Gallic acid. Results & Discussion: The value obtained for Amalaki is 275.57 mg of GA/g equivalent, Ashwagandha is 210.89 mg of GA/g equivalent, Lakshmanphal 73.05 mg of GA/g equivalent Conclusion: Amalaki shown high total phenol content in all three samples, Ashwagandha, Lakshmanphal also rich source of phenols and justifies their use for health benefits.