Hydrogel-based electrochemical LAMP sensor for rapid, contamination-free and ultrasensitive point-of-care detection of Vibrio parahaemolyticus

We report in this work a sure-seal hydrogel-based sensor based on electrochemistry (EC) and loop-mediated isothermal amplification (LAMP) for real-time or end-point monitoring of the amplicon of Vibrio parahaemolyticus. Thanks to the nanoconfinement effect of the porous hydrogel, the LAMP can be significantly accelerated, allowing either real-time or endpoint detection within 15 min with a sensitivity down to 2.11 copies per reaction. A portable device was fabricated by integrating the hydrogel-based EC-LAMP sensor with a flexible and wireless electrochemical system, which enabled the point-of-care detection of Vibrio parahaemolyticus.