拟南芥
生物
脱落酸
转基因
非生物胁迫
转录因子
转基因作物
基因
非生物成分
生物逆境
细胞生物学
植物
遗传学
突变体
古生物学
作者
Mohammed Najib Saidi,Dhawya Mergbi,Amel Souibgui,Inés Yacoubi
标识
DOI:10.1016/j.plaphy.2022.09.027
摘要
Plant-specific NAC transcription factors play important roles in plant development processes, hormone signaling and response to biotic and abiotic stresses. Here, a newly identified membrane-bound NAC gene, designated as TtNTL3A, was isolated from durum wheat. TtNTL3A was homologous to bread wheat TaNAC8 and rice OsNAC8. Moreover, yeast trans-activation assays revealed that TtNTL3A is a transcriptional activator. TtNTL3A was highly expressed in developing seeds and was induced by abiotic stresses, abscisic acid treatment and the infection with Fusarium graminearum. Besides, Transgenic Arabidopsis overexpressing TtNTL3A showed early flowering phenotype and higher tolerance to salt and drought stresses. RT-qPCR analysis revealed that drought and salt-responsive genes were highly expressed in transgenic lines compared to WT plants. Besides, these lines showed resistance to Fusarium graminearum, which was accompanied by a higher expression of pathogenesis-related genes (AtPR-1, Atpdf1.2, and AtNPR1) in TtNTL3A-OE lines. These findings suggest that TtNTL3A is an interesting target of genetic engineering to improve wheat tolerance to biotic and abiotic stresses.
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