纳米探针
光热治疗
化学
下调和上调
体内
信使核糖核酸
纳米技术
癌症治疗
体外
癌症研究
生物物理学
细胞生物学
癌症
生物化学
纳米颗粒
基因
材料科学
生物
生物技术
遗传学
作者
Wenfei Guo,Xiaonan Gao,Qin Zhang,Bo Hu,Jie Sun,Xingya Zhao,Kehua Xu,Bo Tang
标识
DOI:10.1021/acs.analchem.2c05293
摘要
Although photothermal therapy (PTT) has been widely applied for tumor treatment, tumor cells thermotolerance still limits PTT efficiency. Since the overexpressed HSP90α in tumor cells further enhances thermotolerance and protects them from PTT damage, a new nanoprobe that can specifically detect and downregulate HSP90α mRNA was developed to enhance the PTT effect. Based on the HSP90α mRNA sequence, the nanoprobe Au-DNA1/DNA2 can specifically bind to HSP90α mRNA for recovering its fluorescence and further inhibit the synthesis of HSP90α to reduce tumor heat tolerance. Moreover, another nanoprobe, Au-DNA3, can self-assemble with the Au-DNA1 nanoprobe after the detection to form Au aggregations to enhance PTT afterward for better efficiency. Simultaneously, such a design improves tissue penetration and tumor retention, thereby reducing the damage to the surrounding normal tissues. Both in vitro and in vivo experiments showed that the nanoprobes have excellent tumor diagnosis and cancer treatment capabilities, which is of great significance for clinical translational applications.
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