Analysis of Expression Profile of OsUgp1 Gene by Promoter-GUS Chimeric Expression

Promoter of one UDP-glucose pyrophosphorylase gene(OsUgp1) was cloned,fused with β-glucuronidase(GUS) reporter gene and introduced intro rice.Histochemical analysis of GUS activity was used to detect the GUS expression in transgenic rice.The result showed that GUS expressed in root,stem,leave,palea,lemma,ovary,stigma,anther at meiosis stage,developing endosperm and embryo.The expression of OsUgp1 gene in developing seed was compared with that of OsUgp2 gene.An eminent difference between OsUgp1 gene and OsUgp2 gene was found.OsUgp1 gene expressed in endosperm and embryo in the seed 5-20 d after flowering,but OsUgp2 gene almost did not express.The number and location of the cis-element related to endosperm-specific expression were analyzed in the two promoter regions,and a possible molecular mechanism of the divergent expression of two homologous genes was discussed.