Percoll公司
外周血单个核细胞
抗原
免疫放射分析
孵化
凝血活酶
组织因子
化学
分子生物学
凝结
生物
免疫学
体外
生物化学
放射免疫分析
医学
内科学
作者
John C. Giddings,Franco Piovella,Ricetti M,A. Jarvis,Ian R. Peake,A.L. Bloom
出处
期刊:Clinical and Laboratory Haematology
[Wiley]
日期:1980-06-01
被引量:20
标识
DOI:10.1111/j.1365-2257.1980.tb00815.x
摘要
Human peripheral blood leucocytes were examined for their ability to synthesize procoagulant activity in tissue culture. A method was developed utilizing silica coated with polyvinylpyrrolidone (Percoll) to separate monocytes from lymphocytes prior to culture. Coagulation activity was demonstrated in all mononuclear cell cultures after 24 hours incubation. This activity was inhibited by phospholipase C which suggested that tissue thromboplastin was the major source of activity. Specific immunoradiometric assays failed to demonstrate synthesis of factor VIII related antigen (FVIIIRAG) or factor VIII coagulant antigen. The results indicate that under conditions of this study factor VIII was not synthesized in mononuclear cell cultures.
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