费斯特共振能量转移
计算生物学
计算机科学
生物物理学
化学
生物
物理
荧光
光学
作者
Alen Piljić,Carsten Schultz
摘要
The function of many sensors for measuring intracellular events is based on Förster resonance energy transfer (FRET). Here we demonstrate for the first time the use of multiple ratiometric FRET sensors in parallel through spatial and spectral resolution. We monitored three calcium-dependent signaling events by a cytosolic sensor for calcium/calmodulin-dependent protein kinase IIalpha, a membrane-bound sensor for protein kinase C, and a translocating FRET probe based on annexin A4. This multiparameter imaging approach gives insight into the precise timing of cellular events within one single cell, thereby providing a major advantage over single-parameter protocols. This type of imaging will likely be important for high content cell analysis and screening efforts in the future.
科研通智能强力驱动
Strongly Powered by AbleSci AI