肝胰腺
小虾
碱性磷酸酶
等电点
十足目
对虾
生物化学
唾液酸
等电聚焦
生物
酸性磷酸酶
聚丙烯酰胺凝胶电泳
斑节对虾
甲壳动物
化学
酶
色谱法
渔业
作者
Nin‐Nin Chuang,S. L. Shih
出处
期刊:The journal of experimental zoology
[Wiley]
日期:1990-10-01
卷期号:256 (1): 1-7
被引量:37
标识
DOI:10.1002/jez.1402560102
摘要
Abstract Alkaline phosphatase purified from the hepatopancreas of Penaeus japonicus is stable to heating at 65°C for 5 min. The specific activity of the purified enzyme is 25,000 units/mg of protein. After polyacrylamide gel electrophoresis under denaturing conditions, the purified alkaline phosphatase from shrimp was found to consist of deglycosylated monomers of Mr 40,000 and to retain the attachment sites for both sialic acid and phosphatidylinositol. The alkaline phosphatase from shrimp has an isoelectric point (pI) of 7.6 and becomes more alkaline after the removal of either sialic acid or phosphatidylinositol residues.
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