生物
豆类
根瘤
腿血红蛋白
运输机
固氮
慢生型大豆根瘤菌
生物化学
聚磷酸盐
血管组织
磷酸盐
共生
植物
基因
根瘤菌科
细菌
遗传学
作者
Lü Qin,Jing Zhao,Jichun Tian,Liyu Chen,Zhaoan Sun,Yongxiang Guo,Xiuyun Lu,Min Gu,Guohua Xu,Hong Liao
出处
期刊:Plant Physiology
[Oxford University Press]
日期:2012-06-27
卷期号:159 (4): 1634-1643
被引量:154
标识
DOI:10.1104/pp.112.199786
摘要
Abstract Legume biological nitrogen (N) fixation is the most important N source in agroecosystems, but it is also a process requiring a considerable amount of phosphorus (P). Therefore, developing legume varieties with effective N2 fixation under P-limited conditions could have profound significance for improving agricultural sustainability. We show here that inoculation with effective rhizobial strains enhanced soybean (Glycine max) N2 fixation and P nutrition in the field as well as in hydroponics. Furthermore, we identified and characterized a nodule high-affinity phosphate (Pi) transporter gene, GmPT5, whose expression was elevated in response to low P. Yeast heterologous expression verified that GmPT5 was indeed a high-affinity Pi transporter. Localization of GmPT5 expression based on β-glucuronidase staining in soybean composite plants with transgenic roots and nodules showed that GmPT5 expression occurred principally in the junction area between roots and young nodules and in the nodule vascular bundles for juvenile and mature nodules, implying that GmPT5 might function in transporting Pi from the root vascular system into nodules. Overexpression or knockdown of GmPT5 in transgenic composite soybean plants altered nodulation and plant growth performance, which was partially dependent on P supply. Through both in situ and in vitro 33P uptake assays using transgenic soybean roots and nodules, we demonstrated that GmPT5 mainly functions in transporting Pi from roots to nodules, especially under P-limited conditions. We conclude that the high-affinity Pi transporter, GmPT5, controls Pi entry from roots to nodules, is critical for maintaining Pi homeostasis in nodules, and subsequently regulates soybean nodulation and growth performance.
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