三肽
纳米技术
抗菌剂
自愈水凝胶
肽
材料科学
环丙沙星
微生物学
化学
生物化学
抗生素
高分子化学
生物
作者
Silvia Marchesan,Yue Qu,Lynne J. Waddington,Christopher D. Easton,Veronica Glattauer,Trevor Lithgow,Keith M. McLean,John S. Forsythe,Patrick G. Hartley
出处
期刊:Biomaterials
[Elsevier]
日期:2013-05-01
卷期号:34 (14): 3678-3687
被引量:163
标识
DOI:10.1016/j.biomaterials.2013.01.096
摘要
This work reports the self-assembly of a sparingly soluble antibiotic (ciprofloxacin) and a hydrophobic tripeptide (DLeu–Phe–Phe) into supramolecular nanostructures that yield a macroscopic hydrogel at physiological pH. Drug incorporation results in modified morphology and rheological properties of the self-assembled hydrogel. These changes can be correlated with intermolecular interactions between the drug and the peptide, as confirmed by spectroscopic analysis (fluorescence, circular dichroism, IR). The drug appears bound within the hydrogel by non-covalent interactions, and retains its activity over a prolonged release timescale. Antimicrobial activity of the ciprofloxacin-peptide self-assembled hydrogel was evaluated against Staphylococcus aureus, Escherichia coli, and a clinical strain of Klebsiella pneumoniae. Interestingly, the peptide hydrogel alone exhibited a mild anti-bacterial activity against Gram-negative bacteria. While toxic to bacteria, no major cytotoxicity was seen in haemolysis assays of human red blood cells or in mouse fibroblast cell cultures. This new approach of drug incorporation into the nanostructure of a simple tripeptide hydrogel by self-assembly may have important applications for cost-effective wound dressings and novel antimicrobial formulations.
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