核酸内切酶
识别序列
限制性酶
DNA甲基转移酶
连接器
DNA
甲基转移酶
回文序列
化学
劈理(地质)
酶
组合化学
生物化学
分子生物学
纳米颗粒
甲基化
胶体金
回文
生物
纳米技术
材料科学
基因组
计算机科学
基因
古生物学
断裂(地质)
操作系统
作者
Guangtao Song,Cuie Chen,Jinsong Ren,Xiaogang Qu
出处
期刊:ACS Nano
[American Chemical Society]
日期:2009-04-27
卷期号:3 (5): 1183-1189
被引量:179
摘要
An enzyme responsive nanoparticle system that uses a DNA−gold nanoparticle (AuNP) assembly as the substrate has been developed for the simple, sensitive, and universal monitoring of restriction endonucleases in real time. This new assay takes advantage of the palindromic recognition sequence of the restriction nucleases and the unique optical properties of AuNPs and is simpler than the procedure previously described by by Xu et al. (Angew. Chem. Int. Ed. Engl. 2007, 46, 3468−3470). Because it involves only one type of ssDNA modified AuNPs, this assay can be directed toward most of the endonucleases by simply changing the recognition sequence found within the linker DNA. In addition, the endonuclease activity could be quantitatively analyzed by the value of the reciprocal of hydrolysis half time (t1/2−1). Furthermore, our new design could also be applied to the assay of methyltransferase activity since the methylation of DNA inhibits its cleavage by the corresponding restriction endonuclease, and thus, this new methodology can be easily adapted to high-throughput screening of methyltransferase inhibitors.
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