Effects of fullerene derivatives on bioluminescence and application for protease detection

The effects of two C60 derivatives: C60[C(COOH)2]3 and C60(NH2)x(OH)y, have been investigated on the bioluminescence of humanized Gaussia luciferase (hGluc) for the first time. Utilizing the dual properties of carboxyl C60 with luminescence quenching and free radicals scavenging, a novel BRET system was constructed for protease detection with high sensitivity.