CpG站点
DNA甲基化
照明菌甲基化试验
甲基化
体育锻炼的表观遗传学
生物
分子生物学
亚硫酸氢盐测序
甲基化DNA免疫沉淀
RNA导向的DNA甲基化
发起人
基因
遗传学
基因表达
作者
Ja Lok Ku,You Kyung Jeon,Jae Gahb Park
出处
期刊:Methods in molecular biology
日期:2011-01-01
卷期号:: 23-32
被引量:41
标识
DOI:10.1007/978-1-61779-316-5_3
摘要
DNA methylation patterns in CpG-rich regions of promoter, CpG islands, are concerned in regulation of gene expression in mammalian cells. Excessive methylation of CpG dinucleotides in promoter represses the gene expression. In cancer, especially, gene silencing is occurred through aberrant methylation in promoter of tumor suppressor genes. Methylation-specific PCR (MSP) is a method for analysis of DNA methylation patterns in CpG islands. For performing MSP, DNA is modified by and PCR performed with two primer pairs, which are detectable methylated and unmethylated DNA, respectively. MSP is a rapid measure for assession of the methylation status in CpG island.
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