牛血清白蛋白
吉布斯自由能
化学
荧光
焓
猝灭(荧光)
接受者
钠
色谱法
热力学
有机化学
凝聚态物理
量子力学
物理
作者
Chunmei Gao,Mingzhu Liu,Jun Chen,Chen Chen
标识
DOI:10.1163/092050610x519462
摘要
Abstract This paper focuses on the interactions between oxidized sodium alginate (OSA) and bovine serum albumin (BSA) at the molecular level with the purpose to provide basic information for optimizing the biological utilization and pharmaceutical applications of OSA. The results revealed that OSA could strongly quench the intrinsic fluorescence of BSA through a static quenching procedure. The thermodynamic parameters, enthalpy change (H) and entropy change (S), were also calculated. The process of binding was a spontaneous process in which Gibbs free energy change was negative. The distances between donor (BSA) and acceptor (OSA) were calculated to be 2.41, 2.54 and 2.84 nm for SA–BSA, 10% OSA–BSA and 30% OSA–BSA systems, based on Förster non-radiative energy transfer theory, respectively. The results of synchronous fluorescence spectra showed that binding of OSA with BSA cannot induce conformational changes in BSA. Meanwhile, the OSA with low degree of oxidization (DO% 30%) was non-cytotoxic. Therefore, OSA could be promising as a bioactive compound carrier. Keywords: OXIDIZED SODIUM ALGINATEBOVINE SERUM ALBUMINFLUORESCENCE QUENCHINGSYNCHRONOUS FLUORESCENCE
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