次氯酸
荧光
化学
体内
罗丹明
内生
荧光寿命成像显微镜
检出限
生物物理学
渗透(战争)
光化学
生物化学
生物
光学
运筹学
物理
工程类
生物技术
色谱法
作者
Jiaojiao Yang,Mengting Fan,Yan Sun,Mengyuan Zhang,Yuting Xue,Dazhi Zhang,Ting Wang,Xiaoyan Cui
标识
DOI:10.1016/j.snb.2019.127652
摘要
Fluorescence imaging with tailored fluorecent probes are vital for the non-invasive analysis of sophisticated activities in the innate immune system. Fluorescent scaffold with deep tissue penetration are also urgently required for fluorescence imaging in vivo. A phosphorus-substituted rhodamine fluorescent probe PR−HOCl was designed with maximal emission wavelength at 730 nm. The developed probe PR−HOCl exhibits fast response (20 s) and high sensitivity towards HOCl (detection limit, 10 nM) with relatively wide linear range (0.15 μM to 13.5 μM) in vitro. Both endogenic and exogenous hypochlorous acid were sensitively monitored with high selectivity by the developed probe PR−HOCl in living RAW 264.7 cells. Substantial tissue penetrating ability of PR−HOCl has been manifested in the fluorescence imaging of endogenous hypochlorous acid in inflamed nude mice model.
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