The correlation between aspirin resistance and P2Y1 gene polymorphism in patients with cardiovascular or cerebrovascular diseases

Objective To investigates a group of 2 candidate single nucleotide polymorphisms in P2Y1 gene in the blood platelet aggregation pathway associated with aspirin resistance (AR).Methods A total of 330 patients in the Emergency wards and Cardiovascular Department of Shanghai Sixth People' s Hospital were enrolled from October 2010 to March 2012.All subjects were examined including blood TC、TG、HDL-C、LDL-C,blood routine,fasting blood glucose (FBG).Following a course of aspirin treatment for at least 7 days,platelet aggregation stimulated by arachidonic acid (AA) and adenosine diphosphate (ADP) was observed,and P2Y1 gene polymorphisms were analyzed by polymerase chain reaction and DNA sequencing assay.Results Of 330 patients,95 (28.79％) had AR,and 235 (71.21％) were sensitive to aspirin.Type 2 diabetes (OR =2.773),hypertension (OR =3.992),and low level of HDL (OR =6.087) were independent risk factors for the development of AR,This SNP comprise a T for C base substitution at position 893 of the P2Y1 gene,and associated with increase in aspirin resistance (OR 3.16、95％ CI 1.36-7.16、P =0.04).Conclusions The type Ⅱ diabetescidence,hypertension and low level of HDL were independent risk factors for the development of AR.There is correlation between mutation of P2Y1 gene (893C ＞ T) and aspirin resistance. Key words: Aspirin resistance;  Gene sequencing;  P2Y1 gene polymorphisms;  Platelet aggregation