[Objective] The aim was to obtain the optimum extraction conditions of total flavones from Dendroalamus latiftorus Munro leaves.[Method] Taking the content of total flavones as the index,the extraction conditions of total flavones from D.latiftorus leaves were studied by uniform design based on extraction rate.[Result] The optimum extraction conditions were as follows: extraction solvent was 54.73% ethanol solution,extraction temperature was 76 ℃,extraction time was 5 min,and the solid-liquid ratio was 1∶8.[Conclusion]Microwave-ultrasonic extraction had high yield,short extraction time,low alcohol consumption and low ultrasonic power,which was an effective method for the extraction of total flavones from D.latiftorus leaves.