瓜尔胶
乙二醇
精子
甘油
化学
抗氧化剂
精子活力
超氧化物歧化酶
低温保存
DNA断裂
食品科学
生物化学
男科
生物
胚胎
细胞凋亡
医学
有机化学
程序性细胞死亡
细胞生物学
作者
Adel Saberivand,Raheleh Mehrabi,Seyedeh Zeinab Peighambarzadeh,Maryam Saberivand,Mahdiye Shirafkan,Tannaz Haghgouei,Fatemeh Sarvarzadeh,Payam Safaei,Marzieh Saki,Koosha Javdaneh Siahpoush,T. Alizadeh
标识
DOI:10.1016/j.anireprosci.2023.107362
摘要
Sperm cryopreservation often reduces sperm quality by forming of intra- and extracellular ice crystals. Various compounds widely used to counteract this effect. The guar gum was considered as an extracellular cryoprotective substance. The present study evaluated the impact of the co-supplementation of guar gum with ethylene glycol or glycerol in the cryopreservation of bull sperm. Four ejaculates from 4 bulls were pooled and divided into ten groups consisting of 4 controls (glycerol 6%, ethylene glycol 6%, glycerol 3.5%, and ethylene glycol 3.5%, and six treatment groups including guar gum in 0.001% and 0.002% alone and or co-supplemented either with 3.5% glycerol or 3.5% ethylene glycol and frozen in liquid nitrogen. The sperm motility, viability, plasma membrane and DNA integrity, apoptotic-like changes, antioxidant capacity (TAC), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities evaluated. The groups contained 3.5% glycerol + 0.001% guar gum, 3.5% ethylene glycol + 0.001% guar gum, and 0.001% guar gum alone showed higher values for live sperm, antioxidant enzymes, membrane integrity, mitochondrial membrane potential (MMP), fertilization, cleavage, and blastocyst rates; and lower values for apoptotic-like changes, H2O2 level, and DNA damage than the control groups. In conclusion, adding guar gum to the bull sperm diluent either alone or combined with glycerol or ethylene glycol ameliorated sperm viability and kinematic parameters and antioxidant capacity while reducing DNA damage and apoptotic-like changes. Guar gum also has improved embryo development. Due to its cost-effectiveness and physicochemical properties, guar gum is a promising supplement for bull sperm cryopreservation.
科研通智能强力驱动
Strongly Powered by AbleSci AI