保幼激素
生物
绝对图塔
RNA干扰
基因
蜕皮激素受体
克鲁佩尔
遗传学
细胞生物学
基因表达
内分泌学
植物
激素
核糖核酸
核受体
幼虫
剑鱼科
转录因子
作者
Xiaodi Wang,Siyan Bi,Yanhong Tang,Guifen Zhang,Cong Huang,Fang‐Hao Wan,Zhichuang Lü,Wanxue Liu
出处
期刊:Open Biology
[The Royal Society]
日期:2023-05-01
卷期号:13 (5)
被引量:2
摘要
Tomato leaf miner, Tuta absoluta (Meyrick), is one of the most destructive quarantine pests globally. It has been confirmed that Krüppel-homologue 1 (kr-h1) plays a key role in the regulation of juvenile hormone (JH). However, it is unclear how kr-h1 regulates the synthesis of JH and its cascade regulation pattern in tomato leaf miner. Here, we obtained the six JH signalling genes (kr-h1, Methoprene-tolerant, Forkhead box O, Juvenile acid methyltransferase, Juvenile hormone esterase and Fatty acid synthase 2), and applied RNA interference to explore the role of kr-h1 and the seven genes (plus Vitellogenin) regulation relationship in T. absoluta. Bioinformatics analysis revealed the structural characteristics of kr-h1 protein and JH receptor Met, which contained eight C2H2 zinc finger structures and three typical domains of the bHLH-PAS family, respectively. The expression levels of Met and Vg were upregulated after RNAi of kr-h1 gene, while the gene levels of JHAMT and FAS2 were downregulated. Furthermore, topical application of JH analogue to second instar larvae could induce the expression of kr-h1 and inhibit the expression of Met. Our study reveals the mechanism by which kr-h1 regulates JH pathway genes, which could be applied to control the growth of tomato leaf miners.
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