补体系统
细胞凋亡
急性肾损伤
体内
炎症
磷脂酶A2
药理学
生物
化学
细胞生物学
免疫学
医学
内科学
生物化学
免疫系统
酶
生物技术
作者
Xin Tang,Tiantian Wei,Mingjing Guan,Peiyun Li,Yajun Pu,Lijun Cheng,Zhifeng Zhou,Ping Fu,Ling Zhang
标识
DOI:10.1016/j.fct.2022.113591
摘要
Acute kidney injury (AKI) is one of common complications of wasp/bee stings. Phospholipase A2 (PLA2) is a vital pathogenic composition of wasp/bee venom. We aimed to investigate the role of complement mediated mitochondrial apoptosis in PLA2 induced AKI.PLA2 induced AKI model was established by injecting PLA2 into via tail vein on mice. The pathological changes and the microstructural changes of kidney, complement activation, inflammation and apoptosis were detected in vitro and in vivo respectively.The results showed that PLA2 induced AKI models were successfully established in vivo and vitro. Compared with control, serum creatinine and urea nitrogen levels were elevated. Complement system activation and mitochondrial damage were observed. Expressions of IL-6, TNF-α, cleaved caspase-3 and cleaved caspase-9, and Bax/Bcl-2 increased in PLA2 induced AKI models. TNF-α/NF-κB signaling pathway activation in AKI models.In the present study, PLA2 induced AKI model was first successfully established to our knowledge. The role of complement mediated mitochondrial apoptosis pathway in renal tubular epithelial cells in PLA2 induced AKI were verified in vitro and vivo.
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