In recent years, the research in the field of flavonoids has been deepening, their extraction methods have been updated, and their pharmacological effects have been explored, and many new active effects have been discovered, providing new ideas for related fields. This paper provides a systematic review and elaboration on the basic properties, distribution, extraction methods and application cases of flavonoids, as well as some of the deeply researched pharmacological effects of total flavonoids, and discusses the challenges and prospects of total flavonoids in traditional Chinese medicines nowadays.