细胞外基质
活性氧
细胞生物学
细胞生长
Ⅰ型胶原
细胞外
3T3电池
细胞培养
化学
细胞
细胞迁移
电池类型
分子生物学
生物
生物化学
转染
内分泌学
遗传学
作者
Xiaoling Liu,Xinyu Long,Weiwei Liu,Guo‐Dong Yao,Yanrong Zhao,Toshihiko Hayashi,Shunji Hattori,Hitomi Fujisaki,Toshiyuki Ogura,Shin‐ichi Tashiro,Satoshi Onodera,Masayuki Yamato,Takashi Ikejima
标识
DOI:10.1080/10715762.2018.1478088
摘要
Reactive oxygen species (ROS) participate in various cell responses in association with cell proliferation, migration, differentiation, and death. Extracellular matrix (ECM) serves as cellular microenvironments for many kinds of cells, affecting cell activities. However, whether or not ECM influences cellular ROS levels has not been well studied. In this study, cells are cultured on collagen I molecule-coated (mol. coated) dishes and collagen I fibrous gel-covered (gel) dishes to explore their influence on cell behaviours. We found that the levels of ROS in murine 3T3-L1 preadipocytes increased both in cells on mol. coated and those on the gel. Much higher ROS levels were found in the cells cultured on the gel. Cell proliferation and migration were stimulated to opposite directions between the cells on mol. coated and the cells on gel. ROS in a moderate level were positive regulators in the proliferation and migration of cells on mol. coated; however, ROS in a high level served as negative regulators in the cells on gel. These opposite effects on cell proliferation and migration affected by different ROS levels are in parallel with opposite levels of NF-κB p65 activation.
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