A surface plasmon resonance (SPR) sensor system for the determination of sucrose concentration was constructed with a gold thin film sensing chip. The properties of gold thin film are critical factors in exciting surface plasmon resonance phenomena. Therefore in the present paper, the fabrication conditions of gold thin film were investigated to optimize the SPR phenomena. The optimum thickness was obtained as with resonance angle and good surface roughness limitation. about . The linear resonance angle shifts and rapid response were observed from the sucrose concentrations ranged from 0 to 40wt%.