Influence of Huiyang Shengji Formula on wound healing in rats with diabetic skin ulcer

Objective To investigate the influences of Huiyang Shengji Formula and its disassembled formulas on wound healing in rats with diabetic skin ulcer.Methods SD rats(n = 56) were randomly divided into control group,model group,whole formula group(treated with whole Huiyang Shengji Formula),wenyang group(treated with disassembled formula of warming yang and tonifying qi) and huoxue group(treated with disassembled formula of activating blood and promoting tissue regeneration).The method of intraperitoneal injection of STZ-hormone intervention-skin defect-embedding of plastic ring was used for establishing model of diabetic skin ulcer.All groups were given petrolatum gauzes or corresponding medicated gauzes,and after 3 d,7 d,10 d and 14 d,wound healing rate was calculated and wound morphologic changes were observed.After 14 d,the wound granulation tissue was collected from all groups and given HE staining and Masson staining for observing the structure changes of granulation tissue and collagen distribution.Results The wound healing rate was lower in model group than that in control group(P 0.01) after 3d,7d,10 d and 14 d.After 3d,wound healing rate was higher in wenyang group than that in model group,whole formula group and huoxue group(P 0.05).After 7d,10 d and 14 d wound healing rate was higher in all medicinal groups than that in model group(P 0.05).After 7 d and 14 d,wound healing rate was higher in whole formula group than that in model group,wenyang group and huoxue group(P0.05).The granulation tissue grew vigorously,fibrocytes and capillaries were abundant,and inflammatory cells were less in all medicinal groups.Conclusion Huiyang Shengji Formula can effectively improve wound healing in rats with diabetic skin ulcer.The effect of disassembled formula of warming yang and tonifying qi is significant at early stage of ulcer healing and effect of disassembled formula of activating blood and promoting tissue regeneration is better at late stage.