Pathogenesis-related proteins in grapevines induced by salicylic acid and Botrytis cinerea

The grapevine pathogenesis-related proteins (PRs) were studied in order to determine the spatial localization and the temporal production of these inducible proteins. We used leaves of plants obtained from woody cuttings grown in greenhouse. Elicitations were done either with salicylic acid or with strains of Botrytis cinerea. Several PRs extractable at pH 2.8 were found to accumulate in grapevine leaves after salicylic acid treatment or Botrytis infection (SDS-PAGE, coomassie blue). Elicitation with salicylic acid has induced one new protein at about 32 kDa. Botrytis infection has resulted in the accumulation of four major acid-soluble proteins with apparent molecular weights of 27, 32, 34 and 38 kDa. Immunodetections using antisera raised against the tobacco PR-2 family have shown several bands, particularly two bands at 34 and 36 kDa revealed by the anti-2a and present both with salicylic acid and Botrytis.