抗原
生物
黑色素瘤
人口
免疫原性
滑膜肉瘤
癌症
基因
血清学
抗体
癌症研究
免疫学
病理
医学
肉瘤
遗传学
环境卫生
作者
Özlem Türeci,Uğur Şahin,I Schobert,Michael Koslowski,Holger Schmitt,Hansjörg Schild,Frank Stenner,G. Seitz,Hans‐Georg Rammensee,Michael Pfreundschuh
出处
期刊:PubMed
日期:1996-10-15
卷期号:56 (20): 4766-72
被引量:132
摘要
Using autologous serum for the serological analysis of recombinantly expressed clones (SEREX) from a cDNA derived from a human melanoma, several new melanoma antigens were identified that are immunogenic in the autologous host. Sequence analysis revealed that one of these antigens, HOM-MEL-40, was coded for by the SSX2 gene, which has recently been described to be involved in the t(X;18) translocation of human synovial sarcomas. Expression analysis performed by Northern blot and RT-PCR demonstrated the presence of HOM-MEL-40 transcripts in a significant proportion of human melanomas (50%), colon cancers (25 %), hepatocarcinomas (30%), and breast carcinoma (20%) but not in normal tissues except for testis. Sequence comparison with transcripts cloned from testis ruled out mutations in the melanoma-derived HOM-MEL-40. Antibodies against HOM-MEL-40 were found in 10 of 89 patients with melanoma, including 3 of 8 patients with HOM-MEL-40-positive tumors, but not in 41 apparently healthy controls. In view of the specific expression pattern and immunogenicity in cancer patients, HOM-MEL-40 holds promise as a target for immune interventions in a considerable population of patients with HOM-MEL-40-positive tumors.
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