From mechanism to application: Decrypting light‐regulated denitrifying microbiome through geometric deep learning

Abstract Regulation on denitrifying microbiomes is crucial for sustainable industrial biotechnology and ecological nitrogen cycling. The holistic genetic profiles of microbiomes can be provided by meta‐omics. However, precise decryption and further applications of highly complex microbiomes and corresponding meta‐omics data sets remain great challenges. Here, we combined optogenetics and geometric deep learning to form a discover–model–learn–advance (DMLA) cycle for denitrification microbiome encryption and regulation. Graph neural networks (GNNs) exhibited superior performance in integrating biological knowledge and identifying coexpression gene panels, which could be utilized to predict unknown phenotypes, elucidate molecular biology mechanisms, and advance biotechnologies. Through the DMLA cycle, we discovered the wavelength‐divergent secretion system and nitrate‐superoxide coregulation, realizing increasing extracellular protein production by 83.8% and facilitating nitrate removal with 99.9% enhancement. Our study showcased the potential of GNNs‐empowered optogenetic approaches for regulating denitrification and accelerating the mechanistic discovery of microbiomes for in‐depth research and versatile applications.