[Obejective]The virulence and effects of sublethal dose of phoixm on activities of glutathione s-tranferase,carboxylesterase and acetylcholinesterase from pest insect Pealius mori(Takahashi) were studied to provide references for P. mori(Takahashi) resistance research and suitable application of pesticide. [Method]The sublethal doses of phoxim LC10 , LC30 , LC50 were measured by glass tube residual film. The enzyme solution of P. mori(Takahashi) was extracted by glass BioMasher. The enzyme activities of glutathione s-tranferase, carboxylesterase and acetylcholinesterase of P. mori (Takahashi) was detected by BIO-RAD Multiskan Spectrum. [Result]The enzyme activity of glutathione s-tranferase increased notably as the sublethal doses of phoxim disoposal concentration went up, while the enzyme activities of carboxylesterase and acetylcholinesterase obviously decreased. [Conclusion]The glutathione s-tranferase played a role in the course of phoixm detoxifcation in P. mori (Takahashi).