Determination of Hypoxanthine,Xanthine and Adenine Extracts in Four Animal Drugs by HPLC

Hypoxanthine,xanthine and adenine in some animal drug materials were determined by means of HPLC with methanol/water as the mobile phase and a UV detector.This process of sample preparation was improved and the conditions of HPLC were also optimized.The detection limits of hypoxanthine,xanthine and adenine were 0.012×10-6,0.041×10-6,0.042×10-6 g/mL,respectively.The intra-day precisions(RSD) of hypoxanthine,xanthine and adenine were less than 4.1%,7.3% and 4.5%,respectively.The inter-day precisions(RSD) of hypoxanthine,xanthine and adenine were less than 5.8%,2.9% and 3.1%,respectively.The recovery rates of the four samples with added specimen were in a range of 94% to 107%.