乙酰胆碱
传出神经
神经传递
乙酰胆碱酯酶
体内
纳米传感器
外周神经系统
生物物理学
荧光
化学
临床前影像学
神经科学
生物
神经系统
中枢神经系统
作者
Junfei Xia,Hongrong Yang,Michelle Mu,Nicholas Micovic,Kira E. Poskanzer,James R. Monaghan,Heather A. Clark
标识
DOI:10.1073/pnas.2023807118
摘要
The ability to monitor the release of neurotransmitters during synaptic transmission would significantly impact the diagnosis and treatment of neurological diseases. Here, we present a DNA-based enzymatic nanosensor for quantitative detection of acetylcholine (ACh) in the peripheral nervous system of living mice. ACh nanosensors consist of DNA as a scaffold, acetylcholinesterase as a recognition component, pH-sensitive fluorophores as signal generators, and α-bungarotoxin as a targeting moiety. We demonstrate the utility of the nanosensors in the submandibular ganglia of living mice to sensitively detect ACh ranging from 0.228 to 358 μM. In addition, the sensor response upon electrical stimulation of the efferent nerve is dose dependent, reversible, and we observe a reduction of ∼76% in sensor signal upon pharmacological inhibition of ACh release. Equipped with an advanced imaging processing tool, we further spatially resolve ACh signal propagation on the tissue level. Our platform enables sensitive measurement and mapping of ACh transmission in the peripheral nervous system.
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