检出限
荧光
化学
猝灭(荧光)
荧光团
色谱法
水溶液
尿
尿酸
分析化学(期刊)
生物化学
有机化学
光学
物理
作者
Qutong Zheng,Xiong Li,Long Yu,Di Wu,Changying Yang,Yuxiu Xiao
标识
DOI:10.1016/j.jlumin.2021.118076
摘要
An enzyme-free fluorescent sensing platform based on lanthanide metal-organic framework (Eu-BDC, BDC:1,4-benzenedicarboxylic acid) doped electrospinning fiber membrane ([email protected]) was established for the detection of uric acid (UA) in human urine. The [email protected] has Eu3+ characteristic emission, excellent fluorescence stability in pH 4.5–8.0, appropriate hydrophilicity and good reusability (at least 5 cycles). The fluorescence intensity of [email protected] can be quenched by UA through inner filter effect. The relationship between the quenching efficiency and UA concentration [A] accords with Stern-Volmer formula in the range of 0–200 μM: I0/I = 0.0106 [A]+1.0199 (r = 0.9933), where I0 and I are the fluorescence intensity of [email protected] immersed in water and the UA aqueous solution respectively. The quenching constant is 10.6 × 103 M−1 and the limit of detection is 0.6 μM. The [email protected] fluorescent sensing platform was applied to detect UA in real urine samples. The common substances in human urine have no interference with UA sensing, and the recovery is in the range of 95–110% with relative standard deviation less than 2.0%. The proposed method is simple, robust, inexpensive, and has good selectivity, accuracy and precision for quantification of UA in real urine samples.
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