Multiphoton imaging using a quantitative CMOS camera

In this work, we compare the performance of a quantitative scientific complementary metal-oxide semiconductor (qCMOS) camera to the sCMOS camera for multiphoton imaging of tissue specimens. We find that the qCMOS achieves a signal-to-background ratio that is ~2x and ~1.6x higher than that achieved by the sCMOS for twophoton fluorescence and second-harmonic generation (SHG) imaging, respectively. The field-of-view of the qCMOS camera is noticeably larger at ~1.3x that of the sCMOS. We also confirm that the qCMOS can spatially resolve features as fine as 12.5 μm in 200-μm thick tendon tissue, at a penetration depth of 140 μm, using SHG imaging. Our results highlight the applicability of the qCMOS for some multiphoton imaging applications.