Induction of multiple shoots from stem explants of Chardonny grape.

In order to induce multiple shoots from Chardonny grape(Vitis vinifera)explants,the effects of explants,media,hormone concentrations and dark treatment on the induction of multiple shoots were studied.The shoots were induced only from the stem explants,the induction frequency reached to 17.5%.There was no difference in the average number of shoots a-mong 3 media of MS,NN1969 and B5.The shoots were induced from the stems on the MS medium supplemented with BA and IBA,and there was significant difference in the frequency of shoot induction among different concentration treatments of BA and IBA.However,there was no significant difference in the average number of shoots and most of shoots induced were single shoot,indicated that there was no significant effect of the combination of BA with IBA on the induction of multiple shoots in this study.The significant effect of the combination of BA with NAA on both the induction frequency of shoots and the number of multiple shoots was observed.The highest induction frequency(9.7%)of shoots and the highest average number of shoots(10.1)were induced from the stems on the MS medium supplemented with 3.0 mg·L-1 BA and 0.1 mg·L-1 NAA,the highest number from one stem reached up to 27.The roots were induced from the shoots on the 1/2MS medium supplemented with 0.05 mg·L-1 IAA.