The two-photon microfabrication method, which has been developed over several years, is a new way to produce the real three-dimension microstructure. And now, this technology is recognized as a promising process for the creation of nano- and microscale devices. However, there are still important issues to be settled, one of them is the radial superresolution. The radial superresolution diffraction theory is introduced in the two-photon microfabrication system. This method can improve the radial superresolution of the two-photon microfabrication system. The theoretical analysis of the photosensitive resin is discussed based on the exciting power and concentration of free-radical theory. Experiment results of the two-photon microfabrication method verify the method and show that it can provide weight into the microfabrication system.