化学发光
过氧化氢
鲁米诺
化学
活性氧
氧气
四氢生物蝶呤
光化学
活性氧
核化学
色谱法
有机化学
生物化学
酶
辅因子
作者
Hidetoshi Arakawa,Kôichi Masuda,Nobuhiro Tajima,Masako Maeda
出处
期刊:Luminescence
[Wiley]
日期:2007-01-01
卷期号:22 (3): 245-250
被引量:3
摘要
We developed a novel highly sensitive chemiluminescence (CL) method for BH(4). The principle of the proposed method is based on active oxygen formation induced by 1-methoxy-5-methyl phenazinium methyl sulphate (1-methoxy PMS) in the presence of dissolved oxygen. Furthermore, active oxygen is determined by a CL assay involving the luminol reaction with microperoxidase. In this report, we examined the mechanism of formation and identified the reactive oxygen species derived from BH(4) employing 1-methoxy PMS. Additionally, optimum conditions for the CL assay of BH(4) were established.
科研通智能强力驱动
Strongly Powered by AbleSci AI