The aim of this work is to evaluate the characteristics involved in the image reconstruction of the microPET Focus 120. For this evaluation were used two different phantoms; a miniature hot‐rod Derenzo phantom and a National Electrical Manufacturers Association (NEMA) NU4‐2008 image quality (IQ) phantom. The best image quality was obtained when using OSEM3D as the reconstruction method reaching a spatial resolution of 1.5 mm with the Derenzo phantom filled with 18F. Image quality test results indicate a superior image quality for the Focus 120 when compared to previous microPET models.