Abnormal proinflammatory lipoxygenase Products from Peripheral Mononuclear Cells in Patients with Rheumatoid Arthritis

Mononuclear lymphocytes(MNL)isolated from peripheral blood of patients with rheumatoid arthritis(RA) were stimulated by calcium ionophone A23187.The formation of 5—lipoxygenase products leukotriene B4 (LTB4)AND 6—trans leukotriene B4(6—trans LTB4)by MNL was determinated by reverse phase high per- formence liquid chromatography(RP—HPLC).The levels of arachidonic acid metabolites via 5—lipoxygenase pathway by stimulated MNL of patients with RA were evaluated and compared with the data obtained fron a group of control subjects.Mononuclear lymphocytes from patients synthesized significantly greater amounts of LTB4,6—trans LTB4 than did cells from normal subjects(P0.001).The products of 5—lipoxygenase of MNL from patients with active RA compared with that from patients with non—active RA,the level of LTB4 in the former group was significantly greater than that of the latter group(P0.05).proliminary results indi- cate that LTB4 and 6—LTB4 join the inflammatory actions of RA,LTB4 is a important mediator of inflamma- tion.the results suggest LTB4 take part in immunopathology mecanism of RA through influencing several im- munoactive cells.Other suggest would show that NSAIDS includ not only cycloxygenase inhibitors but also lipoxygenase inhibitors,reducing products of inflammatory mediator LTB4.