Photo-triggered release of an antibiotic from an in situ forming hydrogel for antibacterial wound dressings

Event Abstract Back to Event Photo-triggered release of an antibiotic from an in situ forming hydrogel for antibacterial wound dressings Yue Shi1, Vinh X. Truong1, Ketav Kulkarni2, Yue Qu3, George P. Simon1, Richard L. Boyd4, Patrick Perlmutter2, Trevor Lithgow3 and John S. Forsythe1 1 Monash institute of Medical Engineering, Monash University, Department of Material Science and Engineering, Australia 2 Monash University, Department of Chemistry, Australia 3 Monash University, Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiolog, Australia 4 Monash University, Faculty of Medicine, Nursing and Health Sciences Anatomy and Developmental Biology, Australia Introduction: Hydrogels have been used as advanced wound dressing materials because of their ability to improve healing rates and wound appearance[1],[2]. Bacterial infections are found to be a prevalent and persistent problem during chronic wound healing and therefore antimicrobial dressings that are able to release of antibiotics using an external trigger are highly advantageous as sustained release is suspected of causing bacterial resistance[3],[4]. Using light as a trigger is of a considerable interest due to its non- invasive stimulation[5]. Herein, we present a newly designed antimicrobial hydrogel for ‘spray on’ wound dressing applications in which the timing and amount of antibiotic release can be controlled by UV light. Methodology: A representative antibiotic ciprofloxacin was chemically modified and covalently attached on a clickable PEG hydrogel network through a nitrobenzyl carbamate photolabile group. Both the hydrogel network formation and antibiotic attachment were based on a bio-orthogonal catalyst free strain promoted alkyne-zaide cycloaddition (SPAAC). Characterizations were carried out on the hydrogel mechanical properties and drug release properties. Results and discussion: With antibiotic loaded, the hydrogel maintained good mechanical properties and gelation time making it suitable as a ‘spray on’ wound dressing. NMR results indicated the cleavage of carbamate linkage occurred due to UV irradiation. Both HPLC and bacterial tests further confirmed that the native antibiotic could be cleaved from the hydrogel network using a light stimulus and diffuse into the surrounding environment inhibiting bacteria growth efficiently. The amount of drug released was quantitatively measured by HPLC and that the UV light not only triggered the release but also control the amount by different UV dosage as well. Conclusion: Together with the relative fast gelation time, the results suggest using the photo responsive strategy to deliver antibiotics provides control over the timing and dose making it potentially suitable for external wound dressing applications. References:[1] F. Han, Y. Dong, A. Song, R. Yin and S. Li, "Alginate/chitosan based bi-layer composite membrane as potential sustained-release wound dressing containing ciprofloxacin hydrochloride."Applied Surface Science, 2014, 311, 626-634.[2] L. G. Ovington, "Advances in wound dressings." Clinics in Dermatology, 2007, 25, 33-38.[3] K. Vasilev†, J. Cook and H. J. Griesser, "Antibacterial surfaces for biomedical devices." Expert Review of Medical Devices, 2009, 6, 553-567.[4] A. M. Kloxin, A. M. Kasko, C. N. Salinas and K. S. Anseth, "Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical Properties. " Science, 2009, 324, 59-63. Keywords: Infection, Light, Drug delivery, Bioactive molecule Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biomaterials in wound healing Citation: Shi Y, Truong VX, Kulkarni K, Qu Y, Simon GP, Boyd RL, Perlmutter P, Lithgow T and Forsythe JS (2016). Photo-triggered release of an antibiotic from an in situ forming hydrogel for antibacterial wound dressings. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02181 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Yue Shi Vinh X Truong Ketav Kulkarni Yue Qu George P Simon Richard L Boyd Patrick Perlmutter Trevor Lithgow John S Forsythe Google Yue Shi Vinh X Truong Ketav Kulkarni Yue Qu George P Simon Richard L Boyd Patrick Perlmutter Trevor Lithgow John S Forsythe Google Scholar Yue Shi Vinh X Truong Ketav Kulkarni Yue Qu George P Simon Richard L Boyd Patrick Perlmutter Trevor Lithgow John S Forsythe PubMed Yue Shi Vinh X Truong Ketav Kulkarni Yue Qu George P Simon Richard L Boyd Patrick Perlmutter Trevor Lithgow John S Forsythe Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.