Near-Infrared reflectance spectroscopic data have been collected and treated with chemometric methodology.After taking the first order derivative spectrum,a calibration model for determining the palm oil content in peanut oil samples was established by using partial least squares method(PLS),and the cross validation in chemometrics was used for the model testing.The correlation coefficient was 0.9963and the root mean square error of calibration(RMSEC)was 0.937.This method has been successfully used in the analysis of actual samples with satisfactory results.