Evaluation of assay methods for determining fungal laccase activity

Although there are several kinds of methods for determining fungal laccase activity,none of them have been recognized to be acceptable. Laccase activities obtained from different researches were differently defined and were not comparable with others. Spectrophotometric assay methods for fungal laccase activities of different origins are summarized and analyzed. It is concluded that using ABTS as the substrate to determine laccase activity a reasonable and acceptable. A recommendation of a universal and uniform method for determining fungal laccase activities based on ABTS was recommended.