Effects of Tylophora ovata(Lindl) Hook.ex Steud Alkaloids(TOHa) on Proliferation and Apoptosis of HeLa Cells in vitro

This study aimed to explore the effect of Tylophora ovata(Lindl.) Hook.ex Steud(TOHa) alkaloids on proliferation and apoptosis of HeLa cells in vitro.Different concentrations of TOHa were used to treat HeLa cells in vitro for different time.The proliferation was determined by MTT assay,and the cell apoptosis was determined by cell morphology,Annexin V/PI and Giemsa labeling method.It was found experimentally that the TOHa can inhibit the proliferation and activity of Hela cells,and the inhibition effects depend on reaction time and TOHa dose.After being treated with TOHa,Annexin V+/PI-expression of Hela cells rises,and their apoptosis features change accroding to the results of Giemsa staining.