The characteristics of intestinal mucosal barrier in rats resuscitated after hemorrhagic shock

Objective To investigate the characteristics of functional and reconstitution of intestinal mucosal barrier after traumatic-hemorrhagic shock.Methods Specimens on ileum were taken at 0h,1h,3h,6h,12h and 24h after resuscitation.The morphological changes of intestinal mucosa were observed through light and electron microscope.At the same time points,hepato-portal vein blood was collected for endotoxin,and lactulose/mannitol ratio of urine was measured to analyze the change of intestinal mucosal barrier function respectively.Results The apoptosis and necrosis were main manifestations of intestinal mucosal injury after hemorrhagic shock. Most intestinal mucosa was entirely restored at 6h and finished at 12h. The number of goblet cells of intestinal mucosal was reduced. Urine lactulose/mannitol ratio and endotoxin content peaked at 6 h.Conclusion Intestinal mucosal barrier was seriously damaged at the early phase of ischemia-reperfusion injury after hemorrhagic shock, but intestinal mucosa has great repair potential. The restoration of intestinal barrier function is obviously slower than that of morphology.