Expression of NS5ATP13 transregulated by HCV NS5A in Pichia pastoris

Objective To express NS5ATP13 in Pichia pastoris.Methods The constructed plasmid pPICZαA-NS5ATP13 was linarized with Bst XⅠand transformed into Pichia pastoris KM71H,multi-copy recombinants was selected with Zeocin and expressed in BMGY and BMMY media.The Zeocin-resistant clones were induced expression by methanol,which were growing at 30℃ for 4 days.The expressed products were analyzed by SDS-PAGE and Western blot.Results SDS-PAGE and Western blot showed NS5ATP13 protein with expected molecular weight serected in the supernatant.Conclusions NS5ATP13 protein was successfully expressed in Pichia pastoris.