胶质细胞源性神经生长因子
神经营养因子
碱性成纤维细胞生长因子
神经元
生物
干细胞
嗅粘膜
间充质干细胞
神经科学
细胞生物学
神经突
病理
嗅觉系统
生长因子
医学
体外
受体
生物化学
作者
Rafieh Alizadeh,Seyed Kamran Kamrava,Zohreh Bagher,Mohammad Farhadi,Masoumeh Falah,Fatemeh Moradi,Mahdi Eskandarian Boroujeni,Mansooreh Soleimani,Ahmadreza Kamyab,Ali Komeili
标识
DOI:10.1016/j.neulet.2018.12.011
摘要
The production of dopaminergic (DA) neurons from stem cells holds a great promise for future clinical treatment of neurodegenerative diseases, such as Parkinson's disease (PD). Olfactory ecto-mesenchymal stem cells (OE-MSCs) derived from the adult human olfactory mucosa can be easily isolated and expanded in culture while maintaining their immense plasticity. In addition to reduced ethical concerns, OE-MSCs could be transplanted across allogeneic barriers, making them desirable stem cells for clinical applications. The goal of this study was to define the potentiality of human olfactory mucosa-derived MSCs aimed at differentiation into DA neuron-like cells. OE-MSCs were induced to differentiate to DA neuron-like cells in vitro by using sonic hedgehog (SHH), fibroblast growth factor 8 (FGF8), basic fibroblast growth factor (bFGF), Glial cell line-derived neurotrophic factor (GDNF) and brain derived neurotrophic factor (BDNF). Then the differentiated neurons were characterized for expression of DA neuron markers by Real-time PCR, immunocytochemistry and flow cytometry. Our findings showed that differentiated OE-MSCs could significantly express DA neuron markers at mRNA and protein levels along with dopamine release 12 days post-differentiation. These results support the viability and feasibility of using OE-MSCs as a source of in vitro generated DA neuron-like cells for treatment of DA disorders namely PD.
科研通智能强力驱动
Strongly Powered by AbleSci AI