表位
食物过敏
免疫学
医学
免疫疗法
免疫球蛋白E
FOXP3型
人口
抗体
过敏
过敏原
免疫系统
环境卫生
作者
Christine Wai,Nicki Y.H. Leung,Ka Hou Chu,Patrick S.C. Leung
出处
期刊:Methods in molecular biology
日期:2020-11-24
卷期号:: 337-355
被引量:2
标识
DOI:10.1007/978-1-0716-1001-5_21
摘要
Food allergy has been rising in prevalence over the last two decades, affecting more than 10% of the world population. Current management of IgE-mediated food allergy relies on avoidance and rescue medications; research into treatments that are safer and providing guaranteed and durable curative effects is, therefore, essential. T-cell epitope-based immunotherapy holds the potential for modulating food allergic responses without IgE cross-linking. In this chapter, we describe the methods in evaluating the therapeutic capacities of immunodominant T-cell epitopes in animal models of food allergy. Moreover, we explain in detail the methods to measure the allergen-specific antibody levels, prepare single-cell suspension from spleen, and prepare small intestine for immunohistochemical analysis of eosinophils and Foxp3+ cells.
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