脂质体
纳米颗粒
共焦显微镜
药物输送
化学
荧光显微镜
细胞
纳米毒理学
固体脂质纳米粒
单元格排序
细胞毒性
毒品携带者
纳米技术
毒性
生物物理学
细胞生物学
材料科学
体外
生物化学
荧光
生物
物理
有机化学
量子力学
作者
Jamie Strachan,Brendan Dyett,Zeyad Nasa,Céline Valéry,Charlotte E. Conn
标识
DOI:10.1016/j.jcis.2020.05.002
摘要
Cubosomes form part of the next generation of lipid nanoparticle drug delivery vehicles, enabling higher drug encapsulation efficiency, particularly for lipophilic drugs, compared to traditional liposome formulations. However, the mechanism of interaction of cubosome lipid nanoparticles with cells and their resultant cytotoxicity is not yet well characterised. We hypothesise that the uptake mechanism is dependent on the cell-type, and that cellular toxicity will be controlled by both the lipid composition and the uptake mechanism. The uptake of cubosomes into fibroblast and macrophage cell lines was investigated using live-cell imaging on a confocal microscope. Toxicity of the lipid particles was determined using Fluorescence-Activated Cell Sorting (FACS). Atomic Force Microscopy (AFM) provided an overview of the topography of the surface of individual cells. The cells exhibited a contrast in uptake kinetics depending on cell type attributed to varying uptake mechanisms. Cellular toxicity was dictated more by lipid composition than by the internal particle nanostructure or the uptake mechanism. Surface topography showed many surface ridges in the STO cells which could provide a location for cubosome adhesion prior to uptake. The findings provide a crucial guideline for the future engineering and application of lipid nanoparticles in drug delivery applications.
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