凝聚
双孢蘑菇
麦角甾醇
响应面法
壳聚糖
产量(工程)
化学
食品科学
材料科学
色谱法
生物化学
复合材料
蘑菇
作者
Adenilson Renato Rudke,Sandrina A. Heleno,Isabel P. Fernandes,Miguel A. Prieto,Odinei Hess Gonçalves,Alı́rio E. Rodrigues,Isabel C.F.R. Ferreira,María Filomena Barreiro
标识
DOI:10.1016/j.lwt.2019.01.018
摘要
In the present work microencapsulation was applied to an ergosterol enriched extract (EEE) from Agaricus bisporus L. and pure ergosterol (PE) using a complex coacervation process. The process was optimized following two steps: (i) using response surface methodology to optimize the conditions of temperature (T, 55 to 95 °C), protein-to-chitosan ratio (P/C, 0.5 to 10.5), maximizing yield (Y1, %, w/w), efficiency (Y2, %, w/w) and load (Y3, %, w/w); and (ii) using the dose-response methodology to optimize the ergosterol content (E) at the optimal conditions achieved in (i). The global optimal conditions for both cases were the same (T of 55 °C and a P/C of 0.5), but with maximized responses of Y1 = 75.02%; Y2 = 100.00%, Y3 = 12.78%, and Y1 = 19.19%; Y2 = 100.00%, Y3 = 15.87%, respectively. The effect of increasing ergosterol content led to the reduction of the microencapsulation yield, but with an increase in the ergosterol load and maintenance of the microencapsulation efficiency.
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