核酸凝胶电泳
溴化乙锭
琼脂糖
DNA
化学
凝胶电泳
琼脂糖凝胶电泳
电泳
荧光
颜色标记
色谱法
离心
分子生物学
染色
聚丙烯酰胺凝胶电泳
蛋白质凝胶电泳
生物化学
生物
酶
遗传学
量子力学
物理
作者
Michael R. Green,Joseph Sambrook
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2019-01-01
卷期号:2019 (1): pdb.top100388-pdb.top100388
被引量:27
标识
DOI:10.1101/pdb.top100388
摘要
Electrophoresis through agarose or polyacrylamide gels is used to separate, analyze, identify, and purify DNA fragments. The technique is simple, rapid to perform, and capable of resolving fragments of DNA that cannot be separated adequately by other procedures, such as density gradient centrifugation. The location of bands of DNA within the gel can be determined directly by staining with low concentrations of fluorescent intercalating dyes, such as ethidium bromide or SYBR Gold; bands containing as little as 20 pg of double-stranded DNA can then be detected by direct examination of the gel in ultraviolet (UV) light. If necessary, these bands of DNA can be recovered from the gel.
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